• Featured Product
  • KD/KO Validated

Rubicon Polyclonal antibody

Rubicon Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21444-1-AP

Synonyms

Baron, KIAA0226, RUBCN, Rubicon



Tested Applications

Positive WB detected inmouse liver tissue, A549 cells, HeLa cells
Positive IHC detected inhuman testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21444-1-AP is Rubicon Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rubicon fusion protein Ag13715
Full Name KIAA0226
Calculated molecular weight 972 aa, 109 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC033615
Gene symbol KIAA0226
Gene ID (NCBI) 9711
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Rubicon antibody 21444-1-APDownload protocol
IHC protocol for Rubicon antibody 21444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang
humanWB,IHC

Cell Mol Immunol

PMID: 28392573

Authors - Yushun Wan
  • KD Validated
ratWB,IF

Autophagy

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Authors - Luis Muniz-Feliciano
  • KD Validated

Genes Dev

USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.

Authors - Daichao Xu
humanWB

Autophagy

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou