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ATG13 Polyclonal antibody

ATG13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18258-1-AP

Synonyms

ATG13, Autophagy related protein 13, KIAA0652



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse thymus tissue, human brain tissue, mouse cerebellum tissue, mouse testis tissue, BGC-823 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18258-1-AP targets ATG13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG13 fusion protein Ag13090
Full Name KIAA0652
Calculated molecular weight 57 kDa
Observed molecular weight 57-63 kDa
GenBank accession numberBC001331
Gene symbol KIAA0652
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
WB protocol for ATG13 antibody 18258-1-APDownload protocol
IHC protocol for ATG13 antibody 18258-1-APDownload protocol
IF protocol for ATG13 antibody 18258-1-APDownload protocol
IP protocol for ATG13 antibody 18258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int Immunopharmacol

10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells.

Authors - Mengmeng You
mouse

Autophagy

The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.

Authors - Wan Yun Ho
humanWB

J Clin Invest

p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease.

Authors - Zhengwei Ma
mouseWB

Cell

Noncanonical autophagy promotes the visual cycle.

Authors - Kim Ji-Young JY
  • KD Validated