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  • Featured Product
  • KD/KO Validated

Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23333-1-AP

Synonyms

EG5, HKSP, KIF11, kinesin family member 11, Kinesin like protein 1, Kinesin like protein KIF11, KNSL1, TR interacting protein 5, TRIP 5, TRIP5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Eg5 Polyclonal antibody (23333-1-AP)

    WB analysis using 23333-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23333-1-AP (Eg5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using Eg5 Polyclonal antibody (23333-1-AP)

    IP experiment of Raji using 23333-1-AP

    IP result of anti-Eg5 (IP:23333-1-AP, 4ug; Detection:23333-1-AP 1:1000) with Raji cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human lung cancer using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human endometrial cancer using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human lung cancer using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human prostate hyperplasia using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human prostate hyperplasia using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Eg5 Polyclonal antibody (23333-1-AP)

    IHC staining of human endometrial cancer using 23333-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Polyclonal antibody (23333-1-AP)

    IF Staining of HepG2 using 23333-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (23333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Polyclonal antibody (23333-1-AP)

    IF Staining of HepG2 using 23333-1-AP

    Immunofluorescent analysis of HepG2 cells using 23333-1-AP (Eg5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23333-1-AP targets Eg5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Eg5 fusion protein Ag18695
Full Name kinesin family member 11
Calculated Molecular Weight 1056 aa, 119 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC136474
Gene Symbol KIF11
Gene ID (NCBI) 3832
RRIDAB_2716831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Protocols

Product Specific Protocols
WB protocol for Eg5 antibody 23333-1-APDownload protocol
IHC protocol for Eg5 antibody 23333-1-APDownload protocol
IF protocol for Eg5 antibody 23333-1-APDownload protocol
IP protocol for Eg5 antibody 23333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Int J Oncol

Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway

Authors - Ting Xue
View Article
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
View Article
humanWB

Int J Biol Sci

KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.

Authors - Dang Wei
View Article
humanWB

Mol Carcinog

Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.

Authors - Benfang Wang
  • KD Validated
View Article
humanWB

Am J Transl Res

Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.

Authors - Xishan Li
View Article
humanWB,IHC

Neurooncol Adv

KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.

Authors - Ernest Terribas
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Iram (Verified Customer) (01-15-2021)

Eg5 antibody giving a very clean bands and good staining

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
  • Cell Tissue Type: Human colon cancer cells and tissue