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KRAS-2B-specific Polyclonal antibody

KRAS-2B-specific Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18294-1-AP

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pancreas tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human stomach cancer using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human stomach cancer using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human stomach cancer using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IHC staining of human stomach cancer using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KRAS-2B-specific Polyclonal antibody (18294-1-AP)

    IF Staining of HepG2 using 18294-1-AP

    Immunofluorescent analysis of HepG2 cells, using KRAS antibody 18294-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive IHC detected inhuman pancreas tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18294-1-AP targets KRAS-2B-specific in IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, 2A and 2B. The antibody is specific to KRAS, can’t recognize NRAS and HRAS.

Protocols

Product Specific Protocols
IHC protocol for KRAS-2B-specific antibody 18294-1-APDownload protocol
IF protocol for KRAS-2B-specific antibody 18294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols