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Cytokeratin 10-specific Polyclonal antibody

Cytokeratin 10-specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 16855-1-AP

Synonyms

Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytokeratin 10-specific, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    WB analysis using 16855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of mouse skin using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of mouse skin using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of rat skin using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of rat skin using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of human breast cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    IHC staining of human breast cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 10-specific Polyclonal antibody (16855-1-AP)

    FC experiment of A431 using 16855-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human Cytokeratin 10-specific (16855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16855-1-AP targets Cytokeratin 10-specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 10
Calculated Molecular Weight 59 kDa
GenBank Accession NumberNM_000421
Gene Symbol KRT10
Gene ID (NCBI) 3858
RRIDAB_10792092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
IHC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
FC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols