Cytokeratin 17 Polyclonal antibody
Cytokeratin 17 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC,ELISA
Cat no : 18502-1-AP
|Positive WB detected in||HeLa cells, A431 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervical cancer tissue, human colon cancer tissue, human pancreas cancer tissue, human lung cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18502-1-AP is Cytokeratin 17 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 17 fusion protein Ag13351|
|Full Name||keratin 17|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC011901|
|Gene ID (NCBI)||3872|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.
What is the molecular weight of cytokeratin 17?
The molecular weight of cytokeratin 17 is approximately 49 kDa.
What is the function of cytokeratin 17?
Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).
What is the cellular localization of cytokeratin 17?
Cytokeratin 17 expression is located in the cytoplasm.
What is the tissue specificity of cytokeratin 17?
Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.
What is the role of cytokeratin 17 in disease?
Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).
|Product Specific Protocols|
|WB protocol for Cytokeratin 17 antibody 18502-1-AP||Download protocol|
|IHC protocol for Cytokeratin 17 antibody 18502-1-AP||Download protocol|
|IF protocol for Cytokeratin 17 antibody 18502-1-AP||Download protocol|
|IP protocol for Cytokeratin 17 antibody 18502-1-AP||Download protocol|
|FC protocol for Cytokeratin 17 antibody 18502-1-AP||Download protocol|
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