Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18708-1-AP

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18



Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, HepG2 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human colon cancer tissue, human endometrial cancer tissue, human liver tissue, human skin cancer tissue, human breast cancer tissue, human nephroblastoma tissue, human ovary tumor tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18708-1-AP targets Cytokeratin 18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol KRT18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. This antibody is specifically agianst KRT18.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 18708-1-APDownload protocol
IHC protocol for Cytokeratin 18 antibody 18708-1-APDownload protocol
IF protocol for Cytokeratin 18 antibody 18708-1-APDownload protocol
IP protocol for Cytokeratin 18 antibody 18708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols