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Cytokeratin 2e Polyclonal antibody

Cytokeratin 2e Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21725-1-AP

Synonyms

CK 2e, Cytokeratin 2e, Epithelial keratin 2e, K2e, keratin 2, Keratin 2 epidermis, Keratin 2e, KRT2, KRT2A, KRT2E, KRTE, Type II keratin Kb2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    WB analysis using 21725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    WB analysis of A431 using 21725-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    IHC staining of human cervical cancer using 21725-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    IHC staining of human cervical cancer using 21725-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    IHC staining of human oesophagus cancer using 21725-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 2e Polyclonal antibody (21725-1-AP)

    IHC staining of human oesophagus cancer using 21725-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, rat skin tissue.
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21725-1-AP targets Cytokeratin 2e in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 2e fusion protein Ag16384
Full Name keratin 2
Calculated Molecular Weight 639 aa, 66 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC096294
Gene Symbol KRT2
Gene ID (NCBI) 3849
RRIDAB_2878914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 2 is a type II cytokeratin. It is found largely in the upper spinous layer of epidermal keratinocytes, and also in other regions, notably the epidermis covering the knee, thigh, and groin.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 2e antibody 21725-1-APDownload protocol
IHC protocol for Cytokeratin 2e antibody 21725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols