KRT2 Polyclonal antibody

KRT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21725-1-AP

Synonyms

CK 2e, Cytokeratin 2e, Epithelial keratin 2e, K2e, keratin 2, Keratin 2 epidermis, Keratin 2e, KRT2, KRT2A, KRT2E, KRTE, Type II keratin Kb2



Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21725-1-AP is KRT2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT2 fusion protein Ag16384
Full Name keratin 2
Calculated molecular weight 639 aa, 66 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC096294
Gene symbol KRT2
Gene ID (NCBI) 3849
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 2 is a type II cytokeratin. It is found largely in the upper spinous layer of epidermal keratinocytes, and also in other regions, notably the epidermis covering the knee, thigh, and groin.

Protocols

Product Specific Protocols
WB protocol for KRT2 antibody 21725-1-APDownload protocol
IHC protocol for KRT2 antibody 21725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols