Cytokeratin 7-specific Polyclonal antibody
Cytokeratin 7-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17513-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue, human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IHC | See 12 publications below |
| IF | See 5 publications below |
Product Information
17513-1-AP targets Cytokeratin 7-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | keratin 7 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | NM_005556 |
| Gene symbol | KRT7 |
| Gene ID (NCBI) | 3855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 7-specific antibody 17513-1-AP | Download protocol |
| IHC protocol for Cytokeratin 7-specific antibody 17513-1-AP | Download protocol |
| IF protocol for Cytokeratin 7-specific antibody 17513-1-AP | Download protocol |
| IP protocol for Cytokeratin 7-specific antibody 17513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Med FasL on decidual macrophages mediates trophoblast apoptosis: A potential cause of recurrent miscarriage. | ||
Neuroreport Noninvasive urine-derived cell lines derived from neurological genetic patients. | ||
Biomed Pharmacother Leukemia inhibitory factor promote trophoblast invasion via urokinase-type plasminogen activator receptor in preeclampsia. | ||
Int J Clin Exp Pathol Pregnancy-associated plasma protein A up-regulated by progesterone promotes adhesion and proliferation of trophoblastic cells. | ||
Oncol Rep Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model. |
