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Cytokeratin 7-specific Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 17513-1-AP

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Con: 22 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Cytokeratin 7-specific Antibody WB A431 cells 17513-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 17513-1-AP( Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Cytokeratin 7-specific Antibody IHC human breast cancer tissue 17513-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP( Cytokeratin 7-specific antibody at dilution of 1:50 (under 10x lens)

Cytokeratin 7-specific Antibody IHC human breast cancer tissue 17513-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP( Cytokeratin 7-specific antibody at dilution of 1:50 (under 40x lens)

Cytokeratin 7-specific Antibody IF HepG2 cells 17513-1-AP

Immunofluorescent analysis of HepG2 cells using 17513-1-AP(Cytokeratin 7-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 7-specific Antibody IP HepG2 cells 17513-1-AP

IP Result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, HeLa cells, HepG2 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human breast cancer tissue, human ovary tumor tissue, human lung cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
keratin 7
Calculated molecular weight:
51 kDa
Observed molecular weight:
51 kDa
GenBank accession number:
NM_005556
Gene ID (NCBI):
3855
Gene symbol
KRT7
Synonyms
CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.


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