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LAMC1 Monoclonal antibody

LAMC1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G2G4

Cat no : 67706-1-Ig

Synonyms

LAMB2, LAMC1, Laminin 1 subunit gamma, Laminin 10 subunit gamma, Laminin 11 subunit gamma, Laminin 2 subunit gamma, Laminin 3 subunit gamma, Laminin 4 subunit gamma, Laminin 6 subunit gamma, Laminin 7 subunit gamma, Laminin 8 subunit gamma, Laminin 9 subunit gamma, Laminin B2 chain, Laminin subunit gamma 1, S LAM gamma, S laminin subunit gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMC1 Monoclonal antibody (67706-1-Ig)

    WB analysis using 67706-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using LAMC1 Monoclonal antibody (67706-1-Ig)

    WB analysis of human placenta using 67706-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using LAMC1 Monoclonal antibody (67706-1-Ig)

    WB analysis of LNCaP using 67706-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat rectum tissue using LAMC1 Monoclonal antibody (67706-1-Ig)

    WB analysis of rat rectum using 67706-1-Ig

    rat rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig rectum tissue using LAMC1 Monoclonal antibody (67706-1-Ig)

    WB analysis of pig rectum using 67706-1-Ig

    pig rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LAMC1 Monoclonal antibody (67706-1-Ig)

    IF Staining of HeLa using 67706-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LAMC1 antibody (67706-1-Ig, Clone: 1G2G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, LNCaP cells, rat rectum tissue, pig rectum tissue, HepG2 cells, JAR cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67706-1-Ig targets LAMC1 in WB, IF, ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LAMC1 fusion protein Ag14674
Full Name laminin, gamma 1 (formerly LAMB2)
Calculated Molecular Weight 178 kDa
Observed Molecular Weight200-220 kDa
GenBank Accession NumberBC015586
Gene Symbol LAMC1
Gene ID (NCBI) 3915
RRIDAB_2882897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LAMC1 antibody 67706-1-IgDownload protocol
IF protocol for LAMC1 antibody 67706-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuroscience

Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.

Authors - Dan Liu
View Article
humanWB

Rheumatology (Oxford)

The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies

Authors - Yongxin Mo
View Article