Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84474-7-RR targets CD107b / LAMP2 in IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated Molecular Weight | 46 kDa |
| GenBank Accession Number | NM_001017959.2 |
| Gene Symbol | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| RRID | AB_3743563 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P17047-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107b / LAMP2 antibody 84474-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for 84474-7-RR is from a researcher who used the antibody for Western Blot and Immunofluorescence on fibroblast cells at a 1:1000 dilution (Lot 23013900). They reported that lysosomes were clearly labelled in cultured fibroblasts, and bands were observed up to 100 kDa in Western Blot. The reviewer gave the product a 5-star rating, indicating strong satisfaction with its performance across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Durand (Verified Customer) (07-22-2026) | Lysosome are labelled in fibroblasts culture cells. Bands observed to 100 kDa in western blot.
|
