LAMP3 Antibody

LAMP3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12632-1-AP

Synonyms

CD208, DC LAMP, DCLAMP, LAMP, LAMP 3, LAMP3, Protein TSC403, TSC403



Tested Applications

Positive WB detected inmouse lung tissue, A375 cells, A549 cells, mouse thymus tissue, rat lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12632-1-AP targets LAMP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMP3 fusion protein Ag3325
Full Name lysosomal-associated membrane protein 3
Calculated molecular weight 416aa,44 kDa
Observed molecular weight 70 kDa
GenBank accession number BC032940
Gene symbol LAMP3
Gene ID (NCBI) 27074
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Protocols

Product Specific Protocols
WB protocol for LAMP3 antibody 12632-1-APDownload protocol
IHC protocol for LAMP3 antibody 12632-1-APDownload protocol
IP protocol for LAMP3 antibody 12632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

Autophagy activation is required for influenza A virus-induced apoptosis and replication.

Authors - B Yeganeh
humanIF

Stem Cells Int

Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions.

Authors - Yujia Wang
humanIF

Am J Physiol Lung Cell Mol Physiol

Suppression of Influenza A Virus Replication in Human Lung Epithelial Cells by Non-cytotoxic Concentrations Bafilomycin A1.

Authors - Behzad Yeganeh
humanIHC

Am J Pathol

The immune response in non-metastatic axillary lymph nodes is associated with the presence of axillary metastasis and breast cancer patient outcome.

Authors - Carlos López
human

J Alzheimers Dis

Diminished parkin solubility and co-localization with intraneuronal amyloid-β are associated with autophagic defects in Alzheimer's disease.

Authors - Irina Lonskaya
humanWB

Protein Cell

Regeneration of functional alveoli by adult human SOX9+ airway basal cell transplantation.

Authors - Qiwang Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (08-19-2019)

very good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse lung tissues
No image submitted with review