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LCK Polyclonal antibody

LCK Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 24497-1-AP

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using LCK Polyclonal antibody (24497-1-AP)

    WB analysis of mouse thymus using 24497-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24497-1-AP (LCK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LCK Polyclonal antibody (24497-1-AP)

    WB analysis of Jurkat using 24497-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24497-1-AP (LCK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24497-1-AP is LCK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCK fusion protein Ag21475
Full Name lymphocyte-specific protein tyrosine kinase
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_2879574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LCK antibody 24497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols