LGR5 Antibody

LGR5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21833-1-AP

Synonyms

FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5



Tested Applications

Positive IHC detected inhuman small intestine tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

The 21833-1-AP immunogen is Fusion Protein LGR5 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGR5 fusion protein Ag16389
Full Name leucine-rich repeat-containing G protein-coupled receptor 5
Calculated molecular weight 907aa,100 kDa
Observed molecular weight
GenBank accession number BC096324
Gene symbol LGR5
Gene ID (NCBI) 8549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.

Authors - Ajay Kumar Gautam

Oncol Lett

Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.

Authors - Rei Ishibashi

Tumour Biol

Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas.

Authors - Yan Wang
humanIHC

Oncotarget

LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma.

Authors - Gabriella Cunha Vieira