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LINGO1 Polyclonal antibody

LINGO1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 19097-1-AP

Synonyms

LERN1, LINGO1, LRRN6A, UNQ201

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LINGO1 Polyclonal antibody (19097-1-AP)

    WB analysis of mouse brain using 19097-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LINGO1 Polyclonal antibody (19097-1-AP)

    WB analysis of mouse brain using 19097-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using LINGO1 Polyclonal antibody (19097-1-AP)

    WB analysis of rat brain using 19097-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using LINGO1 Polyclonal antibody (19097-1-AP)

    FC experiment of SH-SY5Y using 19097-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug LINGO1 antibody (19097-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19097-1-AP targets LINGO1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LINGO1 fusion protein Ag13553
Full Name leucine rich repeat and Ig domain containing 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC011057
Gene Symbol LINGO1
Gene ID (NCBI) 84894
RRIDAB_10638484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).

Protocols

Product Specific Protocols
WB protocol for LINGO1 antibody 19097-1-APDownload protocol
FC protocol for LINGO1 antibody 19097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols