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LMNB2 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10895-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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LMNB2 Antibody IHC human colon cancer tissue 10895-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

LMNB2 Antibody IF HepG2 cells 10895-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10895-1-AP (LMNB2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

LMNB2 Antibody WB Jurkat cells 10895-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10895-1-AP(LMNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

LMNB2 Antibody IHC human lung cancer tissue 10895-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

LMNB2 Antibody IP Jurkat cells 10895-1-AP

IP Result of anti-LMNB2 (IP:10895-1-AP, 3ug; Detection:10895-1-AP 1:500) with Jurkat cells lysate 2400ug.

LMNB2 Antibody FC HEK-293T cells 10895-1-AP

1X10^6 HEK-293T cells were stained with 0.2ug LMNB2 antibody (10895-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, mouse brain tissue
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human lung cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, SH-SY5Y cells
Positive FC detected in:
HEK-293T cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
LMNB2 fusion protein Ag1335
Full name:
lamin B2
Calculated molecular weight:
68 kDa
Observed molecular weight:
66-68 kDa
GenBank accession number:
BC006551
Gene ID (NCBI):
84823
Gene symbol
LMNB2
Synonyms
LAMB2, lamin B2, LMN2, LMNB2

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