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WB analysis using 10895-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10895-1-AP (LMNB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10895-1-AP
WB result of Lamin B2 antibody (10895-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected HepG2 cells.
IP experiment of Jurkat using 10895-1-AP
IP result of anti-Lamin B2 (IP:10895-1-AP, 3ug; Detection:10895-1-AP 1:500) with Jurkat cells lysate 2400ug.
IHC staining of human colon cancer using 10895-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10895-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10895-1-AP (LMNB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10895-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10895-1-AP (Lamin B2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10895-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10895-1-AP (Lamin B2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Caco-2 using 10895-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Lamin B2 antibody (10895-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IF Staining of SH-SY5Y using 10895-1-AP
Immunofluorescent analysis of SH-SY5Y cells, using LMNB2 antibody 10895-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).
IF Staining of HepG2 using 10895-1-AP
Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody 10895-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

Lamin B2 Polyclonal antibody

Name: Lamin B2 Polyclonal antibody
Brand: Proteintech
SKU: 10895-1-AP
Rating: 4.00 (1 reviews)

Lamin B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10895-1-AP

Publications(29)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (29)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, Raji cells, mouse brain tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Caco-2 cells, SH-SY5Y cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 27 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

10895-1-AP targets Lamin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin B2 fusion protein Ag1335 Predict reactive species
Full Name	lamin B2
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	66-68 kDa
GenBank Accession Number	BC006551
Gene Symbol	Lamin B2
Gene ID (NCBI)	84823
RRID	AB_2136411
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q03252
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Protocols

Product Specific Protocols
WB protocol for Lamin B2 antibody 10895-1-AP	Download protocol
IHC protocol for Lamin B2 antibody 10895-1-AP	Download protocol
IF protocol for Lamin B2 antibody 10895-1-AP	Download protocol
IP protocol for Lamin B2 antibody 10895-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Clin Transl Med Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness. Authors - Lanqing Wang View Article
human	WB	Aging (Albany NY) MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma. Authors - Zongpu Zhang View Article
mouse	WB	J Drug Target Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling. Authors - Minjuan Wang View Article
mouse	WB	Ecotoxicol Environ Saf Antagonizing effects of curcumin against mercury-induced autophagic death and trace elements disorder by regulating PI3K/AKT and Nrf2 pathway in the spleen. Authors - Guifang Zhao View Article
human	WB	Front Oncol Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a. Authors - Fangzhu Wan View Article
human	WB,IF	Oncogenesis Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation. Authors - T Kuga KD Validated View Article