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LMNB2 Polyclonal antibody

LMNB2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10895-1-AP

Synonyms

LAMB2, lamin B2, LMN2, LMNB2



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, SH-SY5Y cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10895-1-AP targets LMNB2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMNB2 fusion protein Ag1335
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 66-68 kDa
GenBank accession numberBC006551
Gene symbol LMNB2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LMNB2 antibody 10895-1-APDownload protocol
IHC protocol for LMNB2 antibody 10895-1-APDownload protocol
IF protocol for LMNB2 antibody 10895-1-APDownload protocol
IP protocol for LMNB2 antibody 10895-1-APDownload protocol
FC protocol for LMNB2 antibody 10895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Drug Target

Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signaling.

Authors - Minjuan Wang
mouseWB

Chem Biol Interact

HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.

Authors - Xiaoyan Dang
ratWB

Chem Biol Interact

Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation.

Authors - Yazhi Fan
humanWB,IF

Exp Cell Res

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Authors - Kuga Takahisa T
humanWB

Biochem J

HIV-1 Tat potently stabilizes Mdm2 and enhances viral replication.

Authors - Rameez Raja
mouseWB

Eur J Immunol

Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2.

Authors - Ralf Willebrand