LPAR4 Polyclonal antibody

LPAR4 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 22165-1-AP

Synonyms

G protein coupled receptor 23, GPR23, LPA 4, LPA receptor 4, LPA4, LPAR4, P2RY9, P2Y purinoceptor 9, P2Y5 LIKE, P2Y5 like receptor, P2Y9, Purinergic receptor 9



Tested Applications

Positive WB detected inSKOV-3 cells, mouse brain tissue, HL-60 cells, 3T3-L1 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22165-1-AP is LPAR4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPAR4 fusion protein Ag17817
Full Name lysophosphatidic acid receptor 4
Calculated Molecular Weight 370 aa, 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC069996
Gene Symbol LPAR4
Gene ID (NCBI) 2846
RRIDAB_11182491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPAR4 antibody 22165-1-APDownload protocol
IHC protocol for LPAR4 antibody 22165-1-APDownload protocol
FC protocol for LPAR4 antibody 22165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation

Authors - Chengsheng Wu