Filter: AllWBIPIHCIF/ICC

WB analysis using 15082-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15082-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of LPS treated HEK-293 using 15082-1-AP
LPS treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15082-1-AP
IP result of anti-LPCAT2 (IP:15082-1-AP, 4ug; Detection:15082-1-AP 1:5000) with HeLa cells lysate 1280 ug.
IHC staining of mouse brain using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thyroid gland using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thyroid gland using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 15082-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 15082-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LPCAT2 antibody (15082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

LPCAT2 Polyclonal antibody

LPCAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15082-1-AP

Publications(1)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

1-acylglycerol-3-phosphate O-acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase, 1-AGP acyltransferase 11, 1-AGPAT 11, 1-alkenylglycerophosphocholine O-acyltransferase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	rat thyroid gland tissue, human rectal cancer tissue, mouse brain tissue, mouse small intestine tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

15082-1-AP targets LPCAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LPCAT2 fusion protein Ag7156 Predict reactive species
Full Name	lysophosphatidylcholine acyltransferase 2
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC002472
Gene Symbol	LPCAT2
Gene ID (NCBI)	54947
RRID	AB_2135557
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7L5N7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPCAT2 antibody 15082-1-AP	Download protocol
IHC protocol for LPCAT2 antibody 15082-1-AP	Download protocol
IF protocol for LPCAT2 antibody 15082-1-AP	Download protocol
IP protocol for LPCAT2 antibody 15082-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Antioxidants (Basel) Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis Authors - Melanie Merkel View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Melanie (Verified Customer) (04-22-2022)

LPCAT2 antibody was diluted 1:1500 in 5% milk in TBST and applied to protein samples from Hek293T cells. The bands were very clear. The antibody could have been diluted even more.

Applications: Western Blot
Primary Antibody Dilution: 1:1500
Cell Tissue Type: Hek293T cells

Christian (Verified Customer) (12-17-2018)

10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C. Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.LPCAT2 siRNA was used to check antibody specificity.The antibody performed excellently, will try at a dilution of 1:800 next time.