Proteintech sets the benchmark for antibody validation.
Either we internally have validated the specificity of this antibody using siRNA KD
and/or there are published KD/KO data for this antibody (see Publications tab)
10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C.
Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.
LPCAT2 siRNA was used to check antibody specificity.
The antibody performed excellently, will try at a dilution of 1:800 next time.