LRP2-Specific Polyclonal antibody

LRP2-Specific Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19700-1-AP

Synonyms

DBS, Fx1a, Glycoprotein 330, gp330, LRP 2, LRP2, Megalin



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, human brain tissue, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

19700-1-AP targets LRP2-Specific in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name low density lipoprotein-related protein 2
Calculated molecular weight 522 kDa
Observed molecular weight 280 kDa
GenBank accession numberNM_004525
Gene symbol LRP2
Gene ID (NCBI) 4036
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS). This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein (PMID: 16638803; 8483924).

Protocols

Product Specific Protocols
WB protocol for LRP2-Specific antibody 19700-1-APDownload protocol
IHC protocol for LRP2-Specific antibody 19700-1-APDownload protocol
FC protocol for LRP2-Specific antibody 19700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Cell Sci

Evidence for a regulated Ca2+ entry in proximal tubular cells and its implication in calcium stone formation.

Authors - Cliff-Lawrence Ibeh
humanWB,IHC

Nephrol Dial Transplant

Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.

Authors - Hiwa Fatah
ratWB

Am J Physiol Lung Cell Mol Physiol

TGF-β inhibits alveolar protein transport by promoting shedding, regulated intramembrane proteolysis and transcriptional downregulation of megalin.

Authors - Luciana C Mazzocchi
ratIHC

Int J Pharm

Kidney targeted delivery of asiatic acid using a FITC labeled renal tubular-targeting peptide modified PLGA-PEG system.

Authors - Junqiu He
mouseWB

Mol Ther Nucleic Acids

Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.

Authors - Bohong Cen
mouseIHC,IF

Metabolism

Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.

Authors - Hitomi Otomo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse Kidney Tissue
LRP2-Specific Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse Kidney Tissue (Cat no:19700-1-AP)
FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse Kidney Tissue