Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19700-1-AP targets LRP2-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | low density lipoprotein-related protein 2 |
| Calculated Molecular Weight | 522 kDa |
| Observed Molecular Weight | 522 kDa |
| GenBank Accession Number | NM_004525 |
| Gene Symbol | LRP2 |
| Gene ID (NCBI) | 4036 |
| RRID | AB_10640428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98164 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| IHC protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| WB protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Megalin (LRP2) antibody, catalog no. 19700-1-AP, has 24 total citations. Journals include Nature Communications, Journal of Advanced Research, Metabolism, Advanced Science, EMBO Reports, ACS Applied Materials & Interfaces, JCI Insight, Nephrology Dialysis & Transplantation, Development, and others. Research fields span renal biology (acute kidney injury, diabetic nephropathy, proteinuria), endosomal recycling, renal-targeted drug delivery, cardiology, lipid metabolism, autophagy, ion channel function, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Mechanism and regulation of cargo entry into the Commander endosomal recycling pathway | ||
J Adv Res Cadmium targeting MLKL-Drp1 axis to trigger mitochondrial oxidative stress contributes to necroinflammation in rat kidney | ||
Metabolism Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy. | ||
Adv Sci (Weinh) Loss of Hepatic Angiotensinogen Attenuates Diastolic Dysfunction in Heart Failure with Preserved Ejection Fraction | ||
J Hazard Mater Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits | ||
Int J Nanomedicine Chitosan Oligosaccharide-Functionalized Ruthenium-Curcumin Nanodots for Targeted Therapy of Acute Kidney Injury. |
Reviews
What customers say
Both reviews (5/5 stars) are from the same user who used this antibody for immunohistochemistry on paraffin-embedded mouse kidney tissue at a 1:100 dilution. The reviewer describes it as an excellent IHC antibody, noting successful results using acidic antigen retrieval and DAB substrate development. Overall, users report strong performance for IHC applications with clear staining in mouse kidney sections.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Brittney (Verified Customer) (11-29-2018) | Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.
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Brittney (Verified Customer) (11-29-2018) | Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.
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