Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse testis tissue, human placenta tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10128-2-AP targets LRRC1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0175 Product name: Recombinant human LRRC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-266 aa of BC003193 Sequence: SIDKRHCSLVYVPEEIYRYARSLEELLLDANQLRELPEQFFQLVKLRKLGLSDNEIQRLPPEIANFMQLVELDVSRNEIPEIPESISFCKALQVADFSGNPLTRLPESFPELQNLTCLSVNDISLQSLPENIGNLYNLASLELRENLLTYLPDSLTQLRRLEELDLGNNEIYNLPESIGALLHLKDLWLDGNQLSELPQEIGNLKNLLCLDVSENRLERLPEEISGLTSLTDLVISQNLLETIPDGIGKLK Predict reactive species |
| Full Name | leucine rich repeat containing 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC003193 |
| Gene Symbol | LRRC1 |
| Gene ID (NCBI) | 55227 |
| RRID | AB_2137458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRRC1 consists of 524 amino acids including 16 leucine-rich repeats and a LAP-specific domain. It has been shown to be highly expressed in several human cancers and can participate in the malignant process of cancer cells, such as hepatocellular carcinoma, breast cancer and non-small cell lung cancer. (PMID: 36370237, PMID: 37505155)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| IHC protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| WB protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LRRC1 (Cat# 10128-2-AP) has 4 total citations published in Molecular Cell, Cells, International Journal of Molecular Sciences, and Journal of Molecular Histology. Research fields include NADPH-glutathione metabolism and ferroptosis, AKT/GSK3β/β-catenin/VEGFA signaling in hepatocellular carcinoma angiogenesis, Smad2/3-mediated liver fibrosis, and β-catenin/c-Myc signaling in acute myeloid leukemia progression.
| Species | Application | Title |
|---|---|---|
Cells LRRC1 Promotes Angiogenesis Through Regulating AKT/GSK3β/β-Catenin/VEGFA Signaling Pathway in Hepatocellular Carcinoma.
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Int J Mol Sci The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3
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J Mol Histol LRRC1 knockdown downregulates MACF1 to inhibit the malignant progression of acute myeloid leukemia by inactivating β-catenin/c-Myc signaling
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