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LY6E Polyclonal antibody
LY6E Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22144-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, Mouse samples.
| Tested Reactivity | human, Mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LY6E fusion protein Ag17573 |
| Full Name | lymphocyte antigen 6 complex, locus E |
| Calculated Molecular Weight | 131 aa, 14 kDa |
| Observed Molecular Weight | 12-16 kDa |
| GenBank Accession Number | BC119708 |
| Gene Symbol | LY6E |
| Gene ID (NCBI) | 4061 |
| RRID | AB_2879007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LY6E antibody 22144-1-AP | Download protocol |
| IHC protocol for LY6E antibody 22144-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Lab Chip LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier | ||
Carcinogenesis α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer. | ||
J Virol LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.
| ||
