|Positive WB detected in||Daudi cells, HeLa cells, HEK-293 cells, rat liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10337-1-AP targets MAD2L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAD2L1 fusion protein Ag0307|
|Full Name||MAD2 mitotic arrest deficient-like 1 (yeast)|
|Calculated molecular weight||24 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC000356|
|Gene ID (NCBI)||4085|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.
|Product Specific Protocols|
|WB protocol for MAD2L1 antibody 10337-1-AP||Download protocol|
|IHC protocol for MAD2L1 antibody 10337-1-AP||Download protocol|
|IF protocol for MAD2L1 antibody 10337-1-AP||Download protocol|
|IP protocol for MAD2L1 antibody 10337-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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