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MAD2L1 Antibody

MAD2L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10337-1-AP

Synonyms

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1



Tested Applications

Positive WB detected inDaudi cells, HeLa cells, HEK-293 cells, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10337-1-AP targets MAD2L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L1 fusion protein Ag0307
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession number BC000356
Gene symbol MAD2L1
Gene ID (NCBI) 4085
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for MAD2L1 antibody 10337-1-APDownload protocol
IHC protocol for MAD2L1 antibody 10337-1-APDownload protocol
IF protocol for MAD2L1 antibody 10337-1-APDownload protocol
IP protocol for MAD2L1 antibody 10337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biometals

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Authors - Jieyuan Wu
humanWB

Oncogene

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu
mouseWB

Sci Adv

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Authors - Changyin Zhou
humanIHC

Oncol Lett

Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors.

Authors - Nakano Yusuke Y
humanIHC

Exp Ther Med

Expression of mitotic-arrest deficiency 2 predicts the efficacy of neoadjuvant chemotherapy for locally advanced uterine cervical cancer.

Authors - Morishita Masanari M
humanIHC

APMIS

Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma.

Authors - Yu Ling L