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MAD2L1BP Polyclonal antibody

MAD2L1BP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15344-1-AP

Synonyms

Caught by MAD2 protein, CMT2, KIAA0110, MAD2L1 binding protein, MAD2L1BP, RP1 261G23.6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAD2L1BP Polyclonal antibody (15344-1-AP)

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAD2L1BP Polyclonal antibody (15344-1-AP)

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAD2L1BP Polyclonal antibody (15344-1-AP)

    WB analysis of HeLa using 15344-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MAD2L1BP Polyclonal antibody (15344-1-AP)

    WB analysis of K-562 using 15344-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using MAD2L1BP Polyclonal antibody (15344-1-AP)

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MAD2L1BP Polyclonal antibody (15344-1-AP)

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using MAD2L1BP Polyclonal antibody (15344-1-AP)

    IHC staining of human colon using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using MAD2L1BP Polyclonal antibody (15344-1-AP)

    IHC staining of human colon using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAD2L1BP Polyclonal antibody (15344-1-AP)

    IF Staining of A431 using 15344-1-AP

    Immunofluorescent analysis of A431 cells using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, K-562 cells, HeLa cells, A549 cells, HepG2 cells
Positive IHC detected inhuman skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15344-1-AP targets MAD2L1BP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L1BP fusion protein Ag7550
Full Name MAD2L1 binding protein
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC002904
Gene Symbol MAD2L1BP
Gene ID (NCBI) 9587
RRIDAB_10836940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

Protocols

Product Specific Protocols
WB protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IHC protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IF protocol for MAD2L1BP antibody 15344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols