MAP2 Polyclonal antibody

MAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17490-1-AP

Synonyms

MAP 2, MAP2, MAP2A, MAP2B, MAP2C



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, mouse cerebellum tissue, mouse brain tissue, rat brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, iPS cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17490-1-AP targets MAP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP2 fusion protein Ag11580
Full Name microtubule-associated protein 2
Calculated molecular weight 200 kDa
Observed molecular weight 280 kDa, 70-85 kDa
GenBank accession numberBC038857
Gene symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).


Protocols

Product Specific Protocols
WB protocol for MAP2 antibody 17490-1-APDownload protocol
IHC protocol for MAP2 antibody 17490-1-APDownload protocol
IF protocol for MAP2 antibody 17490-1-APDownload protocol
IP protocol for MAP2 antibody 17490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Med Virol

PMID: 30624794

Authors - Leqiang Sun
mouseIF

Int J Mol Sci

Zn2+ Aggravates Tau Aggregation and Neurotoxicity.

Authors - Xuexia Li
mouseIF,WB

J Cell Biochem

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Authors - Jun Zhong
ratIF

FASEB J

Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth.

Authors - Yifan Wang
ratIF

Biochem Biophys Res Commun

Inhibitory effects of glucagon-like peptide-1 receptor on epilepsy.

Authors - Yuetao Wen
mouseIF

Cell Death Dis

Microglia as modulators of exosomal alpha-synuclein transmission.

Authors - Yun Xia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Dipen (Verified Customer) (07-20-2020)

Not great for Western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hippocampal neurons
MAP2 Antibody Western Blot, validation (1:500 dilution) in Hippocampal neurons (Cat no:17490-1-AP)
FH

Naomi (Verified Customer) (03-09-2020)

I used the rabbit polyclonal anti-MAP2 antibody at 1:500 dilution to in differentiated PC-12 cells. The cells were fixed in 4% PFA in PBS for 12 minutes. 1 hr in blocking buffer, followed by 24 h incubation in primary antibody. MAP2 was expressed in neurites. Works well at this dilution, great images.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: PC-12
FH

Gloria (Verified Customer) (01-30-2020)

Beautiful staining in primary hippocampal and cortical neurons.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250 dilution
  • Cell Tissue Type: Primary hippocampal and cortical neurons
MAP2 Antibody Immunofluorescence, validation (1:250 dilution dilution) in Primary hippocampal and cortical neurons (Cat no:17490-1-AP)
FH

Ricky (Verified Customer) (02-22-2019)

1:5000 dilution is good enough for a clear and sharp image of western blot. I am now using Li-Cor's Odyssey CLX imaging systems . The second antibody is from Li-Cor with 1:5000 dilution. By the way, I also tried to use 1:1000 dilution for my low expression samples, and there was no bad noise backgrounds.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293