MAP2 Polyclonal antibody, PBS Only

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  WB analysis using 17490-1-AP (same clone as 17490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  IP result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:8000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 17490-1-AP (same clone as 17490-1-PBS)

  IP result of anti-MAP2 (IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:10000) with SH-SY5Y cells lysate 1240 ug. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  IF Staining of iPS cells using 17490-1-AP (same clone as 17490-1-PBS)

  Immunofluorescent staining of MAP2 (17490-1-AP, 1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

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  FC experiment of Neuro-2a using 17490-1-AP (same clone as 17490-1-PBS)

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (17490-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 17490-1-PBS in a different storage buffer formulation.

MAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 17490-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Synonyms

MAP 2, MAP-2, MAP2A, MAP2B, MAP2C

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Product Information

17490-1-PBS targets MAP2 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11580

  Product name: Recombinant human MAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 213-559 aa of BC038857

  Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

• Full Name: microtubule-associated protein 2
• Calculated Molecular Weight: 200 kDa
• Observed Molecular Weight: 280 kDa, 70-85 kDa
• GenBank Accession Number: BC038857
• Gene Symbol: MAP2
• Gene ID (NCBI): 4133
• RRID: AB_2137880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11137
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).