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MEK1/2 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 11049-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

MEK1/2 Antibody WB HL-60 cells 11049-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 11049-1-AP(MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MEK1/2 Antibody IP HL-60 cells 11049-1-AP

IP Result of anti-MEK2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug.

MEK1/2 Antibody IF SH-SY5Y cells 11049-1-AP

Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP(MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HL-60 cells, human brain tissue, Jurkat cells, SH-SY5Y cells
Positive IP detected in:
HL-60 cells
Positive IF detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MEK1/2 fusion protein Ag1481
Full name:
mitogen-activated protein kinase kinase 2
Calculated molecular weight:
44 kDa
Observed molecular weight:
44-47 kDa
GenBank accession number:
BC018645
Gene ID (NCBI):
5605
Gene symbol
MAP2K2
Synonyms
ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2

Background

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and Belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.


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