Tested Applications
| Positive WB detected in | A549 cells, HL-60 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11049-1-AP targets MEK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1481 Product name: Recombinant human MAP2K2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC018645 Sequence: MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44-47 kDa |
| GenBank Accession Number | BC018645 |
| Gene Symbol | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2140649 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IHC protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IP protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| WB protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEK1/2 polyclonal antibody (Cat# 11049-1-AP) has accumulated 132 citations across diverse high-impact journals including Nature Communications, Cell Reports, Oncogene, Developmental Cell, Science Advances, Advanced Science, Molecular Cancer, and Gastroenterology. Research fields span oncology (liver, lung, breast, colorectal, gastric, and ovarian cancers), MAPK/ERK signaling pathway biology, drug discovery, neurology, cardiology, immunology, fibrosis, and ferroptosis. The antibody is predominantly used in Western blot applications for human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Mol Cancer CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer | ||
Gastroenterology M6A-mediated upregulation of FZD10 regulates liver cancer stem cells properties and lenvatinib resistance through WNT/β-catenin and Hippo signaling pathways | ||
Cancer Commun (Lond) Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma | ||
Exp Hematol Oncol Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway | ||
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer |
