MEK1/2 Polyclonal antibody
MEK1/2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF,ELISA
Conjugate
Unconjugated
Cat no : 11049-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, human brain tissue, Jurkat cells, SH-SY5Y cells |
Positive IP detected in | HL-60 cells |
Positive IF detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
Product Information
11049-1-AP targets MEK1/2 in WB, IP, IF,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MEK1/2 fusion protein Ag1481 |
Full Name | mitogen-activated protein kinase kinase 2 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 44-47 kDa |
GenBank accession number | BC018645 |
Gene symbol | MAP2K2 |
Gene ID (NCBI) | 5605 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
IF protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
IP protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Oncol Identification of RE1-Silencing Transcription Factor as a Promoter of Metastasis in Pancreatic Cancer. | ||
Mol Med Rep hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas. | ||
Int J Clin Exp Pathol SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway. | ||
Cell Signal Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy. | ||
Front Cell Dev Biol Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma. |