MEK1/2 Polyclonal antibody
MEK1/2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 11049-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, human brain tissue, Jurkat cells, SH-SY5Y cells |
| Positive IP detected in | HL-60 cells |
| Positive IF detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
Product Information
11049-1-AP targets MEK1/2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MEK1/2 fusion protein Ag1481 |
| Full Name | mitogen-activated protein kinase kinase 2 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44-47 kDa |
| GenBank accession number | BC018645 |
| Gene symbol | MAP2K2 |
| Gene ID (NCBI) | 5605 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IF protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IP protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol Identification of RE1-Silencing Transcription Factor as a Promoter of Metastasis in Pancreatic Cancer. | ||
Mol Med Rep hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas. | ||
Int J Clin Exp Pathol SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway. | ||
Cell Signal Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy. | ||
