MEK1/2 Polyclonal antibody

MEK1/2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11049-1-AP

Synonyms

ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2



Tested Applications

Positive WB detected inHL-60 cells, human brain tissue, Jurkat cells, SH-SY5Y cells
Positive IP detected inHL-60 cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

11049-1-AP targets MEK1/2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK1/2 fusion protein Ag1481
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene symbol MAP2K2
Gene ID (NCBI) 5605
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

Protocols

Product Specific Protocols
WB protocol for MEK1/2 antibody 11049-1-APDownload protocol
IF protocol for MEK1/2 antibody 11049-1-APDownload protocol
IP protocol for MEK1/2 antibody 11049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Identification of RE1-Silencing Transcription Factor as a Promoter of Metastasis in Pancreatic Cancer.

Authors - Haoyi Jin
humanWB

Mol Med Rep

hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas.

Authors - Yi Wang
humanWB

Int J Clin Exp Pathol

SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway.

Authors - Xing-Lan Li
humanWB

Int Immunopharmacol

BPTES inhibits anthrax lethal toxin-induced inflammatory response.

Authors - Jinling Wang
humanWB

Cell Signal

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Authors - Lisa M Cooper
humanWB

Cell Death Dis

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Authors - Wenjuan Li