Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, HeLa cells, RAW264.7, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11376 Product name: Recombinant human MAP2K4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-399 aa of BC036032 Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 4 |
| Calculated Molecular Weight | 399 aa, 44 kDa |
| Observed Molecular Weight | 44-50 kDa |
| GenBank Accession Number | BC036032 |
| Gene Symbol | MAP2K4 |
| Gene ID (NCBI) | 6416 |
| RRID | AB_2878389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| WB protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MAP2K4 antibody (Cat# 17340-1-AP) has 19 citations across journals including Metabolism, EBioMedicine, Acta Pharmacol Sinica, J Transl Med, Aging Cell, Biochem Pharmacol, J Hazard Mater, and others. Research fields span cancer biology (gastric, cervical, melanoma, pancreatic, breast, and prostate cancers), liver disease (steatohepatitis, acute liver failure), metabolic disorders (diabetic kidney disease, diabetic retinopathy), pulmonary fibrosis, and MAPK/JNK signaling pathway mechanisms.
| Species | Application | Title |
|---|---|---|
Metabolism The chemokine CCL20 promotes hepatocyte cholesterol deposition during metabolic dysfunction-associated steatohepatitis by regulating OLR1 expression | ||
Chin Herb Med Ganoderic acid a derivative induces apoptosis of cervical cancer cells by inhibiting JNK pathway. | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
Acta Pharmacol Sin Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway
| ||
J Transl Med UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma. |
