Validation Data Gallery
|Sample-dependent, check data in validation data gallery|
14385-1-AP targets ASK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASK1 fusion protein Ag5818|
|Full Name||mitogen-activated protein kinase kinase kinase 5|
|Calculated molecular weight||155 kDa|
|GenBank accession number||BC054503|
|Gene ID (NCBI)||4217|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38.
Thioredoxin-1 phosphorylated at T100 is needed for its anti-apoptotic activity in HepG2 cancer cells.
\Non-haemolytic enterotoxin (Nhe) of Bacillus cereus induces apoptosis in Vero cells.
J Mol Cell Cardiol
Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction.