MAP4K5 Antibody

MAP4K5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12827-1-AP

Synonyms

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5



Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K5 fusion protein Ag3547
Full Name mitogen-activated protein kinase kinase kinase kinase 5
Calculated molecular weight 846aa,95 kDa
Observed molecular weight 58 kDa,95 kDa,120 kDa
GenBank accession number BC036013
Gene symbol MAP4K5
Gene ID (NCBI) 11183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAP4K5 antibody 12827-1-APDownload protocol
IHC protocol for MAP4K5 antibody 12827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols