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MAPK11 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17376-1-AP

Featured Product KD/KO validated

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

MAPK11 Antibody IF HEK-293 cells 17376-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HEK-293 cells using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MAPK11 Antibody WB Jurkat cells 17376-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

MAPK11 Antibody IHC human brain tissue 17376-1-AP

Immunohistochemical of paraffin-embedded human brain using 17376-1-AP(MAPK11 antibody) at dilution of 1:50 (under 40x lens)

MAPK11 Antibody IHC human brain tissue 17376-1-AP

Immunohistochemical of paraffin-embedded human brain using 17376-1-AP(MAPK11 antibody) at dilution of 1:50 (under 10x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, human heart tissue, mouse skeletal muscle tissue
Positive IHC detected in:
human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MAPK11 fusion protein Ag11184
Full name:
mitogen-activated protein kinase 11
Calculated molecular weight:
364aa,41 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
BC027933
Gene ID (NCBI):
5600
Gene symbol
MAPK11
Synonyms
MAP kinase 11, MAP kinase p38 beta, MAPK 11, MAPK11, p38 2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

Background

MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).


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