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MAPK11 Polyclonal antibody

MAPK11 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17376-1-AP

Synonyms

MAP kinase 11, MAP kinase p38 beta, MAPK 11, MAPK11, p38 2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

Validation Data Gallery

  • WB analysis using 17376-1-AP

    WB analysis using 17376-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17376-1-AP

    IHC staining of human brain using 17376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 17376-1-AP

    IHC staining of human brain using 17376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HEK-293 using 17376-1-AP

    IF Staining of HEK-293 using 17376-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, human heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17376-1-AP targets MAPK11 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPK11 fusion protein Ag11184
Full Name mitogen-activated protein kinase 11
Calculated molecular weight 364 aa, 41 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC027933
Gene symbol MAPK11
Gene ID (NCBI) 5600
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).

Protocols

Product Specific Protocols
WB protocol for MAPK11 antibody 17376-1-APDownload protocol
IHC protocol for MAPK11 antibody 17376-1-APDownload protocol
IF protocol for MAPK11 antibody 17376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

Authors - Shutao Zheng
  • KD Validated
View Article
humanWB

Mol Pharmacol

p38β MAPK signaling mediates exenatide-stimulated microglial β-endorphin expression.

Authors - Hai-Yun Wu
  • KD Validated
View Article