Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HeLa cells|
|Positive IHC detected in||human brain tissue, human gliomas tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:300-1:1500|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10274-1-AP targets TAU in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TAU fusion protein Ag0354|
|Full Name||microtubule-associated protein tau|
|Calculated molecular weight||37-46, 79-81 kDa|
|Observed molecular weight||66-70 kDa, 100 kDa|
|GenBank accession number||BC000558|
|Gene ID (NCBI)||4137|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 70-kDa and 100-kDa bands in brain tissues.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (02-14-2019)
The antibody worked well with the mouse primary cells and tissue.