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MAVS; VISA Antibody

MAVS; VISA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14341-1-AP

Synonyms

CARDIF, IPS 1, IPS1, KIAA1271, MAVS, VISA



Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14341-1-AP targets MAVS; VISA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAVS; VISA fusion protein Ag5655
Full Name mitochondrial antiviral signaling protein
Calculated molecular weight 57 kDa
Observed molecular weight 50-55,70-75 kDa
GenBank accession number BC044952
Gene symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or interferon beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Protocols

Product Specific Protocols
WB protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IHC protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IF protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IP protocol for MAVS; VISA antibody 14341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

BMC Microbiol

MiR-3470b promotes bovine ephemeral fever virus replication via directly targeting mitochondrial antiviral signaling protein (MAVS) in baby hamster Syrian kidney cells.

Authors - Peili Hou
WB

Vet Comp Oncol

Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells.

Authors - Masaya Igase
humanWB

Front Pharmacol

Epigoitrin, an Alkaloid From Isatis indigotica, Reduces H1N1 Infection in Stress-Induced Susceptible Model in vivo and in vitro.

Authors - Zhuo Luo
humanWB

Cell Rep

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Authors - Kerong Wang

Oncotarget

MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS).

Authors - Shengfeng Wan
humanWB

J Gen Virol

Porcine Reproductive and Respiratory Syndrome Virus 3C Protease Cleaves MAVS to Antagonize IFN-β Expression.

Authors - Jianming Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Damien (Verified Customer) (09-03-2019)

No convince by this anti-MAVS antibody. In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
No image submitted with review