- Featured Product
- KD/KO Validated
MAVS; VISA Antibody
MAVS; VISA Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14341-1-AP
CARDIF, IPS 1, IPS1, KIAA1271, MAVS, VISA
Validation Data Gallery
|Positive WB detected in||HeLa cells, HuH-7 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14341-1-AP targets MAVS; VISA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAVS; VISA fusion protein Ag5655|
|Full Name||mitochondrial antiviral signaling protein|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||50-55,70-75 kDa|
|GenBank accession number||BC044952|
|Gene ID (NCBI)||57506|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or interferon beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
|Product Specific Protocols|
|WB protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IHC protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IF protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|IP protocol for MAVS; VISA antibody 14341-1-AP||Download protocol|
|Click here to view our Standard Protocols|
MiR-3470b promotes bovine ephemeral fever virus replication via directly targeting mitochondrial antiviral signaling protein (MAVS) in baby hamster Syrian kidney cells.
Vet Comp Oncol
Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells.
Epigoitrin, an Alkaloid From Isatis indigotica, Reduces H1N1 Infection in Stress-Induced Susceptible Model in vivo and in vitro.
The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.
MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS).
J Gen Virol
Porcine Reproductive and Respiratory Syndrome Virus 3C Protease Cleaves MAVS to Antagonize IFN-β Expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Damien (Verified Customer) (09-03-2019)
No convince by this anti-MAVS antibody. In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.