MBD2 Polyclonal antibody

MBD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55200-1-AP

Synonyms

Demethylase, DMTase, MBD2, NY CO 41



Tested Applications

Positive WB detected inJurkat cells, A431 cells, HEK-293 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55200-1-AP targets MBD2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name methyl-CpG binding domain protein 2
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberNM_003927
Gene symbol MBD2
Gene ID (NCBI) 8932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Protocols

Product Specific Protocols
WB protocol for MBD2 antibody 55200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genet Test Mol Biomarkers

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Authors - Mei-Rong Liang