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MBIP Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66102-1-Ig |CloneNo.: 2E6H11

Featured Product KD/KO validated

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Con: 181 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

MBIP Antibody WB HEK-293 cells 66102-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig(MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MBIP Antibody WB HEK-293 cells 66102-1-Ig

WB result of MBIP antibody(66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells

MBIP Antibody IHC human heart tissue 66102-1-Ig

Immunohistochemical analysis of paraffin-embedded human heart slide using 66102-1-Ig( MBIP Antibody) at dilution of 1:50

MBIP Antibody IHC human heart tissue 66102-1-Ig

Immunohistochemical analysis of paraffin-embedded human heart slide using 66102-1-Ig( MBIP Antibody) at dilution of 1:50

MBIP Antibody IP HEK-293 cells 66102-1-Ig

IP Result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.

Species specificity:
human
Positive WB detected in:
293 cells, HepG2 cells, human heart tissue
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human heart, tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MBIP fusion protein Ag18090
Full name:
MAP3K12 binding inhibitory protein 1
Calculated molecular weight:
39 kDa
Observed molecular weight:
39 kDa
GenBank accession number:
BC005197
Gene ID (NCBI):
51562
Gene symbol
MBIP
Synonyms
MBIP, MUK binding inhibitory protein

Background

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.


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