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MBIP Monoclonal antibody

MBIP Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6H11

Cat no : 66102-1-Ig

Synonyms

MBIP, MUK binding inhibitory protein

Validation Data Gallery

  • WB analysis of HEK-293 using 66102-1-Ig

    WB analysis of HEK-293 using 66102-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66102-1-Ig

    WB analysis of HEK-293 using 66102-1-Ig

    WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

  • IHC staining of human heart using 66102-1-Ig

    IHC staining of human heart using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66102-1-Ig (MBIP Antibody) at dilution of 1:50.

  • IHC staining of human heart using 66102-1-Ig

    IHC staining of human heart using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66102-1-Ig (MBIP Antibody) at dilution of 1:50.

  • IP experiment of HEK-293 using 66102-1-Ig

    IP experiment of HEK-293 using 66102-1-Ig

    IP Result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.

Tested Applications

Positive WB detected in293 cells, HepG2 cells, human heart tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart, tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66102-1-Ig is MBIP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MBIP fusion protein Ag18090
Full Name MAP3K12 binding inhibitory protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005197
Gene symbol MBIP
Gene ID (NCBI) 51562
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Protocols

Product Specific Protocols
WB protocol for MBIP antibody 66102-1-IgDownload protocol
IHC protocol for MBIP antibody 66102-1-IgDownload protocol
IP protocol for MBIP antibody 66102-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols