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  • Featured Product
  • KD/KO Validated

MBIP Monoclonal antibody

MBIP Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E6H11

Cat no : 66102-1-Ig

Synonyms

MBIP, MUK binding inhibitory protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MBIP Monoclonal antibody (66102-1-Ig)

    WB analysis using 66102-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MBIP Monoclonal antibody (66102-1-Ig)

    WB analysis of HEK-293 using 66102-1-Ig

    WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

  • Western Blot (WB) analysis of HepG2 cells using MBIP Monoclonal antibody (66102-1-Ig)

    WB analysis of HepG2 using 66102-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MBIP Monoclonal antibody (66102-1-Ig)

    WB analysis of human heart using 66102-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MBIP Monoclonal antibody (66102-1-Ig)

    IP experiment of HEK-293 using 66102-1-Ig

    IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MBIP Monoclonal antibody (66102-1-Ig)

    IHC staining of human kidney using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MBIP Monoclonal antibody (66102-1-Ig)

    IHC staining of human kidney using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MBIP Monoclonal antibody (66102-1-Ig)

    IHC staining of mouse testis using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MBIP Monoclonal antibody (66102-1-Ig)

    IHC staining of mouse testis using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human heart tissue, HepG2 cells, LNCaP cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66102-1-Ig is MBIP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MBIP fusion protein Ag18090
Full Name MAP3K12 binding inhibitory protein 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC005197
Gene Symbol MBIP
Gene ID (NCBI) 51562
RRIDAB_2881501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Protocols

Product Specific Protocols
WB protocol for MBIP antibody 66102-1-IgDownload protocol
IHC protocol for MBIP antibody 66102-1-IgDownload protocol
IP protocol for MBIP antibody 66102-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols