Filter: AllWBIPIHC

WB analysis using 66102-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66102-1-Ig
WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.
WB analysis of human heart using 66102-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66102-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 66102-1-Ig
IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.
IHC staining of human kidney using 66102-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 66102-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 66102-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 66102-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MBIP Monoclonal antibody

Name: MBIP Monoclonal antibody
Brand: Proteintech
SKU: 66102-1-Ig

MBIP Monoclonal Antibody for WB, IP, IHC, ELISA

Cat No. 66102-1-Ig

Clone No.2E6H11

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

MBIP, MUK binding inhibitory protein

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, human heart tissue, HepG2 cells, LNCaP cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:3000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66102-1-Ig targets MBIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	MBIP fusion protein Ag18090 Predict reactive species
Full Name	MAP3K12 binding inhibitory protein 1
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC005197
Gene Symbol	MBIP
Gene ID (NCBI)	51562
RRID	AB_2881501
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NS73
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Protocols

Product Specific Protocols
WB protocol for MBIP antibody 66102-1-Ig	Download protocol
IHC protocol for MBIP antibody 66102-1-Ig	Download protocol
IP protocol for MBIP antibody 66102-1-Ig	Download protocol