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MBIP Monoclonal antibody
MBIP Monoclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2E6H11
Cat no : 66102-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | 293 cells, HepG2 cells, human heart tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human heart, tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 66102-1-Ig is MBIP Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | MBIP fusion protein Ag18090 |
Full Name | MAP3K12 binding inhibitory protein 1 |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC005197 |
Gene symbol | MBIP |
Gene ID (NCBI) | 51562 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.
Protocols
Product Specific Protocols | |
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WB protocol for MBIP antibody 66102-1-Ig | Download protocol |
IHC protocol for MBIP antibody 66102-1-Ig | Download protocol |
IP protocol for MBIP antibody 66102-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |