|Positive WB detected in||HeLa cells, rat kidney tissue, human kidney tissue, mouse kidney tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
13798-1-AP targets MFN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Chicken, human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MFN1 fusion protein Ag4762|
|Full Name||mitofusin 1|
|Calculated molecular weight||741 aa, 84 kDa|
|Observed molecular weight||86 kDa|
|GenBank accession number||BC040557|
|Gene ID (NCBI)||55669|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
J Neurosci Res
Nicotinamide mononucleotide alters mitochondrial dynamics by SIRT3-dependent mechanism in male mice.
J Clin Invest
Restoring mitofusin balance prevents axonal degeneration in a Charcot-Marie-Tooth type 2A model.
CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
Cell Death Dis
OPA1 overexpression ameliorates mitochondrial cristae remodeling, mitochondrial dysfunction, and neuronal apoptosis in prion diseases.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ruiting (Verified Customer) (09-18-2020)
Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.
Kishor (Verified Customer) (01-30-2019)
I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue