Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55030-1-AP targets MKK7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 7 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_145185 |
| Gene Symbol | MKK7 |
| Gene ID (NCBI) | 5609 |
| RRID | AB_2881257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14733 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
MKK7 Polyclonal antibody (55030-1-AP) has 14 citations published in journals including Metabolism, Phytomedicine, Journal of Translational Medicine, Aging Cell, Biochemical Pharmacology, International Immunopharmacology, Frontiers in Physiology, BMC Cancer, PLoS One, Gene, OncoTargets and Therapy, Current Medical Sciences, Chinese Herbal Medicine, and Journal of Agricultural and Food Chemistry. Research fields span diabetes complications, cancer (melanoma, lung, breast, glioma), ferroptosis, pulmonary fibrosis, bone metabolism, and JNK/MAPK signaling pathway regulation.
| Species | Application | Title |
|---|---|---|
Metabolism Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier. | ||
Phytomedicine Ginsenoside compound K alleviates brain aging by inhibiting ferroptosis through modulation of the ASK1-MKK7-JNK signaling pathway | ||
Chin Herb Med Ganoderic acid a derivative induces apoptosis of cervical cancer cells by inhibiting JNK pathway. | ||
J Transl Med UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma. | ||
Aging Cell Lactobacillus paracasei L9 Ameliorates Pulmonary Fibrosis in Aged Mice via Gut-Lung Axis-Mediated Regulation of Immune Cell Migration. | ||
J Agric Food Chem Ginsenoside F4 Alleviates Skeletal Muscle Insulin Resistance by Regulating PTP1B in Type II Diabetes Mellitus |
