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  • KD/KO Validated

MLXIP Polyclonal antibody

MLXIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 13614-1-AP

Synonyms

bHLHe36, KIAA0867, MIR, MLX interacting protein, MLXIP, MONDOA



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13614-1-AP targets MLXIP in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLXIP fusion protein Ag4519
Full Name MLX interacting protein
Calculated molecular weight 919 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC039704
Gene symbol MLXIP
Gene ID (NCBI) 22877
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Protocols

Product Specific Protocols
WB protocol for MLXIP antibody 13614-1-APDownload protocol
IHC protocol for MLXIP antibody 13614-1-APDownload protocol
IF protocol for MLXIP antibody 13614-1-APDownload protocol
IP protocol for MLXIP antibody 13614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Elife

Cellular acidosis triggers human MondoA transcriptional activity by driving mitochondrial ATP production.

Authors - Blake R Wilde
humanIP

JCI Insight

PMID: 31287806

Authors - Byungyong Ahn
humanchIP

Antioxid Redox Signal

Dl-3-n-Butylphthalide Inhibits NLRP3 Inflammasome and Mitigates Alzheimer's-Like Pathology via Nrf2-TXNIP-TrX Axis.

Authors - Chun-Yan Wang
WB

Mol Cell Biol

Ras Suppresses TXNIP Expression by Restricting Ribosome Translocation.

Authors - Zhizhou Ye
mouseWB, IP

Mol Cell Biol

MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction.

Authors - Mohan R Kaadige
  • KO Validated
humanWB

Oncogene

BRCA1 deficiency sensitizes breast cancer cells to bromodomain and extra-terminal domain (BET) inhibition.

Authors - Baoyuan Zhang
  • KD Validated