MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 23898-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11



Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 23898-1-AP is MME,CD10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag20963
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC101658
Gene symbol MME
Gene ID (NCBI) 4311
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for MME,CD10 antibody 23898-1-APDownload protocol
IHC protocol for MME,CD10 antibody 23898-1-APDownload protocol
IF protocol for MME,CD10 antibody 23898-1-APDownload protocol
FC protocol for MME,CD10 antibody 23898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Physiol Biochem

NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.

Authors - Xinliu Zeng