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MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

Cat no : 23898-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    WB analysis of mouse kidney using 23898-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    WB analysis of rat kidney using 23898-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    WB analysis of human kidney using 23898-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    WB analysis of rat kidney using 23898-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    IHC staining of human kidney using 23898-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    IHC staining of human kidney using 23898-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using MME,CD10 Polyclonal antibody (23898-1-AP)

    IF Staining of mouse kidney using 23898-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MME,CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MME,CD10 Polyclonal antibody (23898-1-AP)

    IF Staining of HepG2 using 23898-1-AP

    Immunofluorescent analysis of HepG2 cells, using MME antibody 23898-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using MME,CD10 Polyclonal antibody (23898-1-AP)

    FC experiment of HepG2 using 23898-1-AP

    1X10^6 HepG2 cells were stained with .2ug MME,CD10 antibody (23898-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23898-1-AP is MME,CD10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag20963
Full Name membrane metallo-endopeptidase
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC101658
Gene Symbol MME
Gene ID (NCBI) 4311
RRIDAB_2879354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for MME,CD10 antibody 23898-1-APDownload protocol
IHC protocol for MME,CD10 antibody 23898-1-APDownload protocol
IF protocol for MME,CD10 antibody 23898-1-APDownload protocol
FC protocol for MME,CD10 antibody 23898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Physiol Biochem

NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.

Authors - Xinliu Zeng
View Article
mouseWB

Biochem Cell Biol

IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse.

Authors - Caiyun Li
View Article