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MMP1 Antibody
MMP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10371-2-AP
Synonyms
CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human cervical cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 49 publications below |
| IHC | See 11 publications below |
| IF | See 1 publications below |
Product Information
10371-2-AP targets MMP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MMP1 fusion protein Ag0547 |
| Full Name | matrix metallopeptidase 1 (interstitial collagenase) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 52-56 kDa,62 kDa |
| GenBank accession number | BC013875 |
| Gene symbol | MMP1 |
| Gene ID (NCBI) | 4312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP1 antibody 10371-2-AP | Download protocol |
| IHC protocol for MMP1 antibody 10371-2-AP | Download protocol |
| IF protocol for MMP1 antibody 10371-2-AP | Download protocol |
| IP protocol for MMP1 antibody 10371-2-AP | Download protocol |
| FC protocol for MMP1 antibody 10371-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Ther Med Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity. | ||
Int J Oncol 25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway. | ||
J Exp Clin Cancer Res ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma. | ||
Phytomedicine Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats. | ||
Front Pharmacol Schisandrin A Inhibits the IL-1β-Induced Inflammation and Cartilage Degradation via Suppression of MAPK and NF-κB Signal Pathways in Rat Chondrocytes. | ||
Acta Biochim Biophys Sin (Shanghai) Liraglutide alleviates cardiac fibrosis through inhibiting P4hα-1 expression in STZ-induced diabetic cardiomyopathy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Saravanakumar (Verified Customer) (10-31-2017) | I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.
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