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- KD/KO Validated
MMP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10371-2-AP
CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Validation Data Gallery
|Positive WB detected in||A549 cells, NIH/3T3 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human cervical cancer tissue, rat testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10371-2-AP targets MMP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP1 fusion protein Ag0547|
|Full Name||matrix metallopeptidase 1 (interstitial collagenase)|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||52-56 kDa,62 kDa|
|GenBank accession number||BC013875|
|Gene ID (NCBI)||4312|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).
|Product Specific Protocols|
|WB protocol for MMP1 antibody 10371-2-AP||Download protocol|
|IHC protocol for MMP1 antibody 10371-2-AP||Download protocol|
|IF protocol for MMP1 antibody 10371-2-AP||Download protocol|
|IP protocol for MMP1 antibody 10371-2-AP||Download protocol|
|FC protocol for MMP1 antibody 10371-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Ther Med
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Saravanakumar (Verified Customer) (10-31-2017)
I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.