MMP1 Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 10371-2-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Reviews(1)


-_-

Freight/Packing

Con: 57 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat, rabbit

Positive WB detected in:
A549 cells, NIH/3T3 cells

Positive IP detected in:
A549 cells

Positive IHC detected in:
human cervical cancer tissue, rat testis tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
matrix metallopeptidase 1 (interstitial collagenase)

Calculated molecular weight:
54 kDa

Observed molecular weight:
52-56 kDa,62 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MMP1

Synonyms
CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Background

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

MMP1 Rabbit Polyclonal antibody

Saravanakumar Marimuthu, 10-31-2017

Applications
Western Blot
Lot Number
00046806

Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:2000

Cell/Tissue Type
MCF10A cells


I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.

Back
to top