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MMP1 Antibody

MMP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10371-2-AP

Synonyms

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1



Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman cervical cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10371-2-AP targets MMP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP1 fusion protein Ag0547
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Calculated molecular weight 54 kDa
Observed molecular weight 52-56 kDa,62 kDa
GenBank accession number BC013875
Gene symbol MMP1
Gene ID (NCBI) 4312
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Protocols

Product Specific Protocols
WB protocol for MMP1 antibody 10371-2-APDownload protocol
IHC protocol for MMP1 antibody 10371-2-APDownload protocol
IF protocol for MMP1 antibody 10371-2-APDownload protocol
IP protocol for MMP1 antibody 10371-2-APDownload protocol
FC protocol for MMP1 antibody 10371-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Ther Med

Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.

Authors - Tangshan Zhang
humanWB

Int J Oncol

25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.

Authors - Saisai Wang
humanWB

J Exp Clin Cancer Res

ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.

Authors - Xiaozhi Li
ratWB

Phytomedicine

Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats.

Authors - Huanghe Yu
ratWB

Front Pharmacol

Schisandrin A Inhibits the IL-1β-Induced Inflammation and Cartilage Degradation via Suppression of MAPK and NF-κB Signal Pathways in Rat Chondrocytes.

Authors - Chang Tu
ratWB,IHC

Acta Biochim Biophys Sin (Shanghai)

Liraglutide alleviates cardiac fibrosis through inhibiting P4hα-1 expression in STZ-induced diabetic cardiomyopathy.

Authors - Tong Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Saravanakumar (Verified Customer) (10-31-2017)

I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A cells
No image submitted with review