Tested Applications
| Positive WB detected in | HepG2 cells, SMMC-7721 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11575-1-AP targets MPP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2158 Product name: Recombinant human MPP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-540 aa of BC023638 Sequence: PLGVTFRVENNDLVIARILHGGMIDRQGLLHVGDIIKEVNGHEVGNNPKELQELLKNISGSVTLKILPSYRDTITPQQVFVKCHFDYNPYNDNLIPCKEAGLKFSKGEILQIVNREDPNWWQASHVKEGGSAGLIPSQFLEEKRKAFVRRDWDNSGPFCGTISSKKKKKMMYLTTRNAEFDRHEIQIYEEVAKMPPFQRKTLVLIGAQGVGRRSLKNRFIVLNPTRFGTTVPFTSRKPREDEKDGQAYKFVSRSEMEADIKAGKYLEHGEYEGNLYGTKIDSILEVVQTGRTCILDVNPQALKVLRTSEFMPYVVFIAAPELETLRAMHKAVVDAGITTKLLTDSDLKKTVDESARIQRAYNHYFDLIIINDNLDKAFEKLQTAIEKLRMEPQWVPISWVY Predict reactive species |
| Full Name | membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) |
| Calculated Molecular Weight | 540 aa, 61 kDa |
| Observed Molecular Weight | 55-61 kDa |
| GenBank Accession Number | BC023638 |
| Gene Symbol | MPP6 |
| Gene ID (NCBI) | 51678 |
| RRID | AB_2250848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZW5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MPP6 antibody 11575-1-AP | Download protocol |
| WB protocol for MPP6 antibody 11575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MPP6 antibody (Cat# 11575-1-AP) has 3 total citations published in Frontiers in Immunology, Frontiers in Cell and Developmental Biology, and Journal of Southern Medical University. The associated research fields encompass hepatocellular carcinoma prognosis, immune evasion, angiogenesis, and non-small cell lung cancer biology. All cited studies utilized human samples via WB and/or IHC applications.
| Species | Application | Title |
|---|---|---|
Front Immunol Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma | ||
Front Cell Dev Biol Divergent roles of circMPP6 and its parental gene MPP6 in non-small cell lung cancer. | ||
Nan Fang Yi Ke Da Xue Xue Bao [High expression of MPP6 predicts poor patient prognosis and promotes malignant biological behaviors of hepatocellular carcinoma cells]. |
