MRAS Polyclonal antibody

MRAS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14213-1-AP

Synonyms

M RAs, MRAS, muscle RAS oncogene homolog, R RAS3, Ras related protein M Ras, Ras related protein R Ras3, RRAS3



Tested Applications

Positive WB detected inmouse brain tissue, mouse lung tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14213-1-AP targets MRAS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRAS fusion protein Ag5433
Full Name muscle RAS oncogene homolog
Calculated molecular weight 24 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC047690
Gene symbol MRAS
Gene ID (NCBI) 22808
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRAS antibody 14213-1-APDownload protocol
IHC protocol for MRAS antibody 14213-1-APDownload protocol
IP protocol for MRAS antibody 14213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols