MRPS22 Polyclonal antibody

MRPS22 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10984-1-AP

Synonyms

C3orf5, COXPD5, GIBT, GK002, MRP S22, MRPS22, RPMS22, S22mt



Tested Applications

Positive WB detected inJurkat cells, rat spleen tissue, mouse liver tissue, mouse spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 17 publications below

Product Information

10984-1-AP targets MRPS22 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS22 fusion protein Ag1421
Full Name mitochondrial ribosomal protein S22
Calculated molecular weight 41 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC009296
Gene symbol MRPS22
Gene ID (NCBI) 56945
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPS22, also named as C3orf5, RPMS22 and S22mt, is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). MRPS22 is a 360-amino-acid protein that has been found in a limited number of species, having no ortholog in fungi or prokaryotes. The exact location of MRPS22 in the three dimensional structure of the ribosome and its exact function are currently unknown. Recently, a MRPS22 defect was shown to strongly hamper assembly of the small ribosomal subunit, whereas assembly of the large subunit and part of the small subunit were only mildly affected.(PMID:21189481)

Protocols

Product Specific Protocols
WB protocol for MRPS22 antibody 10984-1-APDownload protocol
IHC protocol for MRPS22 antibody 10984-1-APDownload protocol
IP protocol for MRPS22 antibody 10984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB.

Authors - Fenna Hensen
mouseWB

Cell Rep

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Authors - Jakob D Busch
mouseWB

PLoS One

Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.

Authors - Panagiotis Mitsopoulos
humanWB

FEBS J

Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.

Authors - Francesco Bruni
humanWB

Hum Mol Genet

Mutations in the mitochondrial ribosomal protein MRPS22 lead to primary ovarian insufficiency.

Authors - Anlu Chen
humanWB

Sci Rep

Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure.

Authors - Joachim M Gerhold