• Featured Product
  • KD/KO Validated

MSH2 Antibody

MSH2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15520-1-AP

Synonyms

COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2



Tested Applications

Positive WB detected inHeLa cells, A2780 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15520-1-AP targets MSH2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-105 kDa
GenBank accession numberBC021566
Gene symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
WB protocol for MSH2 antibody 15520-1-APDownload protocol
IHC protocol for MSH2 antibody 15520-1-APDownload protocol
IF protocol for MSH2 antibody 15520-1-APDownload protocol
IP protocol for MSH2 antibody 15520-1-APDownload protocol
FC protocol for MSH2 antibody 15520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Medicine (Baltimore)

A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.

Authors - Lin-Qing Yuan
humanWB

Cell Death Dis

Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.

Authors - Kang Liu
humanIF

Cell Biochem Funct

Quantitative Proteomics Analysis Reveals Nuclear Perturbation in Human Glioma U87 Cells treated with Temozolomide.

Authors - Jinglin Guo
humanWB

Exp Mol Med

PMID: 23143558

Authors - He Yu-qi YQ
humanIHC

Virchows Arch

Significance of p53 expression in background endometrium in endometrial carcinoma.

Authors - Thuy Thi Nguyen
humanIHC

J Surg Oncol

Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.

Authors - Jun Zhu