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  • Western Blot (WB) analysis of HEK-293 cells using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis of HEK-293 using 82845-3-RR

    WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis using 82845-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MSH3 Recombinant antibody (82845-3-RR)

    IP experiment of HeLa using 82845-3-RR

    IP result of anti-MSH3 (IP:82845-3-RR, 4ug; Detection:82845-3-RR 1:3000) with HeLa cells lysate 1320 ug.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U-251 using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U2OS using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MSH3 Recombinant monoclonal antibody

MSH3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82845-3-RR
Clone No.1J23

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

1J23, Divergent upstream protein, DUC1, DUG, hMSH3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHSC-T6 cells, HEK-293 cells, HEK-293 cells, HeLa cells, MOLT-4 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inU-251 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82845-3-RR targets MSH3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18060

Product name: Recombinant human MSH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC130434

Sequence: MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAATAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKL

Predict reactive species
Full Name mutS homolog 3 (E. coli)
Calculated Molecular Weight 1137 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
RRIDAB_3086552
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Protocols

Product Specific Protocols
IF protocol for MSH3 antibody 82845-3-RRDownload protocol
IP protocol for MSH3 antibody 82845-3-RRDownload protocol
WB protocol for MSH3 antibody 82845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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