|Positive WB detected in||HEK-293 cells, human brain tissue, HuH-7 cells, K-562 cells, mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10794-1-AP targets MTHFD1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MTHFD1 fusion protein Ag1225|
|Full Name||methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase|
|Calculated molecular weight||101 kDa|
|Observed molecular weight||101 kDa|
|GenBank accession number||BC009806|
|Gene ID (NCBI)||4522|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2.
Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
Oxidative stress inhibits distant metastasis by human melanoma cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (08-26-2019)