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MTMR8 Polyclonal antibody

MTMR8 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 12299-1-AP

Synonyms

MTMR8, myotubularin related protein 8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of A375 using 12299-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of human placenta using 12299-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MTMR8 Polyclonal antibody (12299-1-AP)

    IF Staining of A375 using 12299-1-AP

    Immunofluorescent analysis of A375 cells using 12299-1-AP (MTMR8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A375 cells, human placenta tissue
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12299-1-AP targets MTMR8 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR8 fusion protein Ag2944
Full Name myotubularin related protein 8
Calculated Molecular Weight 704 aa, 79 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC012399
Gene Symbol MTMR8
Gene ID (NCBI) 55613
RRIDAB_2298075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MTMR8 antibody 12299-1-APDownload protocol
IF protocol for MTMR8 antibody 12299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols