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mTOR Polyclonal antibody

mTOR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20657-1-AP

Synonyms

FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20657-1-AP targets mTOR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, cow, hamster, human, medaka, mouse, pig, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight 250-289 kDa
GenBank accession numberNM_004958
Gene symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). The antibody is specific to MTOR.

Protocols

Product Specific Protocols
WB protocol for mTOR antibody 20657-1-APDownload protocol
IHC protocol for mTOR antibody 20657-1-APDownload protocol
IF protocol for mTOR antibody 20657-1-APDownload protocol
IP protocol for mTOR antibody 20657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

Has_circ_0008274 promotes cell proliferation and invasion involving AMPK/mTOR signaling pathway in papillary thyroid carcinoma.

Authors - G-K Zhou
mouseWB

Drug Des Devel Ther

Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway.

Authors - Yuan Wang
humanWB

Br J Pharmacol

Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage.

Authors - Zhang Mao
ratWB

Food Funct

Isodunnianol alleviates doxorubicin-induced myocardial injury by activating protective autophagy.

Authors - Can Chen
mouseWB

Am J Transl Res

Glycyrrhizic acid ameliorates LPS-induced acute lung injury by regulating autophagy through the PI3K/AKT/mTOR pathway.

Authors - Lihua Qu
ratWB

Cell Tissue Res

Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.

Authors - Rongzong Zheng