MUT Polyclonal antibody

MUT Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17034-1-AP

Synonyms

MCM, Methylmalonyl CoA isomerase, MUT



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, HepG2 cellls
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17034-1-AP targets MUT in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUT fusion protein Ag10523
Full Name methylmalonyl Coenzyme A mutase
Calculated Molecular Weight 750 aa, 83 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC016282
Gene Symbol MUT
Gene ID (NCBI) 4594
RRIDAB_2147263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MUT antibody 17034-1-APDownload protocol
IHC protocol for MUT antibody 17034-1-APDownload protocol
IP protocol for MUT antibody 17034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Hepatology

Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia.

Authors - Randy J Chandler
human,mouseWB

Cells

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Authors - Jana Key
mouseIHC

PLoS One

Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia

Authors - Shengwen Zhang
mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell
humanWB

Biochim Biophys Acta Mol Basis Dis

ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis

Authors - Lan Wang