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  • Featured Product
  • KD/KO Validated

MVP/LRP Polyclonal antibody

MVP/LRP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16478-1-AP

Synonyms

LRP, major vault protein, MVP, MVP/LRP, VAULT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MVP/LRP Polyclonal antibody (16478-1-AP)

    WB analysis using 16478-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    WB analysis of mouse lung using 16478-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using MVP/LRP Polyclonal antibody (16478-1-AP)

    WB analysis of RAW264.7 using 16478-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    WB analysis of rat lung using 16478-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    WB analysis of human brain using 16478-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IP experiment of mouse lung using 16478-1-AP

    IP result of anti-MVP/LRP (IP:16478-1-AP, 4ug; Detection:16478-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human lung cancer using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human breast cancer using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human kidney using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human kidney using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human testis using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human testis using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human skin using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human skin using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human breast cancer using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human breast cancer using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MVP/LRP Polyclonal antibody (16478-1-AP)

    IHC staining of human lung cancer using 16478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MVP/LRP Polyclonal antibody (16478-1-AP)

    IF Staining of HeLa using 16478-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MVP/LRP antibody (16478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human kidney tissue, human testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16478-1-AP targets MVP/LRP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MVP/LRP fusion protein Ag9593
Full Name major vault protein
Calculated Molecular Weight 893 aa, 99 kDa
Observed Molecular Weight 99-104 kDa
GenBank Accession NumberBC015623
Gene Symbol MVP
Gene ID (NCBI) 9961
RRIDAB_2147597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

Protocols

Product Specific Protocols
WB protocol for MVP/LRP antibody 16478-1-APDownload protocol
IHC protocol for MVP/LRP antibody 16478-1-APDownload protocol
IF protocol for MVP/LRP antibody 16478-1-APDownload protocol
IP protocol for MVP/LRP antibody 16478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
View Article
humanWB,IHC,IF,IP

Drug Resist Updat

CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis

Authors - Jing Wu
  • KD Validated
View Article
humanWB

Nat Commun

MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.

Authors - Yun Teng
  • KO Validated
View Article
humanWB

Front Cell Dev Biol

Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.

Authors - Xubin Dong
  • KD Validated
View Article
mouseWB

Front Cell Dev Biol

Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.

Authors - Xinlei Li
View Article
mouseWB

Cell Commun Signal

Differential RNA packaging into small extracellular vesicles by neurons and astrocytes.

Authors - Xuan Luo
View Article