c-Myb Polyclonal antibody

c-Myb Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, co-IP, ELISA

Conjugate

Unconjugated

Cat no : 17800-1-AP

Synonyms

c myb_CDS, Cmyb, c-Myb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17800-1-AP targets c-Myb in WB, IP, co-IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-Myb fusion protein Ag12021
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Calculated molecular weight 640 aa, 72 kDa
Observed molecular weight 72-80 kDa
GenBank accession numberBC064955
Gene symbol MYB
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for c-Myb antibody 17800-1-APDownload protocol
IP protocol for c-Myb antibody 17800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,co-IP

RNA Biol

SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma.

Authors - Xiaoteng Cui